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Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum
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scientific article
2002
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Abstract

A clone encoding farnesyl diphosphate synthase (FPPS) was obtained by PCR from a cDNA library made from young leaves of Artemisia annua. A cDNA clone encoding the tobacco epi‐aristolochene synthase (eAS) was kindly supplied by J. Chappell (University of Kentucky, Lexington, KY, USA). Two fusions were constructed, i.e. FPPS/eAS and eAS/FPPS. The stop codon of the N‐terminal enzyme was removed and replaced by a short peptide (Gly‐Ser‐Gly) to introduce a linker between the two ORFs. These two...

Authors Maria Brodelius, Anneli Lundgren, Per Mercke, Peter E Brodelius
Volume Vol. 269, No. 14, pp. 3570-3577
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